Products > Antibodies > Primary Antibodies > Monoclonal Antibodies

CLIC4 Rabbit mAb (A21149)

Publication (1) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CLIC4 Rabbit mAb (A21149)

Western blot analysis of various lysates, using CLIC4 Rabbit mAb (A21149) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCLIC4 Rabbit mAb
Catalog No.A21149
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2998
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
ImmunogenRecombinant protein of Human CLIC4.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsH1; huH1; p64H1; CLIC4L; MTCLIC
Calculated MW29kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, SW620, HepG2, Mouse lung, Mouse kidney, Rat lung, Rat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle membrane, Mitochondrion, Nucleus matrix, Single-pass membrane protein, centrosome, cytoskeleton, microtubule organizing center
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - CLIC4 Rabbit mAb (A21149)}

Western blot - CLIC4 Rabbit mAb (A21149)

Western blot analysis of various lysates, using CLIC4 Rabbit mAb (A21149) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Related Targets

Based on existing publications, the following genes are closely related to research on CLIC4. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to CLIC4. (Distance between topics and target gene indicate popularity.) CLIC4

* Data provided by citexs.com, for reference only.

Publishing research using A21149? Please let us know so that we can cite the reference in this datasheet.