CLIC4 Rabbit mAb-Monoclonal Antibodies

CLIC4 Rabbit mAb (A21149)

Western blot analysis of various lysates, using CLIC4 Rabbit mAb (A21149) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	CLIC4 Rabbit mAb
Catalog No.	A21149
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2998

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Immunogen information

Immunogen	Recombinant protein of Human CLIC4.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	H1; huH1; p64H1; CLIC4L; MTCLIC
Calculated MW	29kDa
Observed MW	28kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, SW620, HepG2, Mouse lung, Mouse kidney, Rat lung, Rat brain
Cellular location	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle membrane, Mitochondrion, Nucleus matrix, Single-pass membrane protein, centrosome, cytoskeleton, microtubule organizing center
Customer validation	WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Research Area & PathwaysEndocrine & MetabolismMitochondrial metabolismMitochondrial markers

CLIC4 Rabbit mAb images

Western blot - CLIC4 Rabbit mAb (A21149)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

Related Targets

Based on existing publications, the following genes are closely related to research on CLIC4. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to CLIC4. (Distance between topics and target gene indicate popularity.) CLIC4

* Data provided by citexs.com, for reference only.