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CLIC4 Rabbit mAb (A21149)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - CLIC4 Rabbit mAb (A21149)

Western blot analysis of extracts of various cell lines, using CLIC4 antibody (A21149) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCLIC4 Rabbit mAb
Catalog No.A21149
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
ImmunogenRecombinant protein of Human CLIC4.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsCLIC4; CLIC4L; H1; MTCLIC; huH1; p64H1
Calculated MW28kDa
Observed MW28KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, SW620, HepG2, Mouse lung, Mouse kidney, Rat lung, Rat brain
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle membrane, Mitochondrion, Nucleus matrix, Single-pass membrane protein, centrosome, cytoskeleton, microtubule organizing center
ABclonal:Western blot - CLIC4 Rabbit mAb (A21149)}

Western blot - CLIC4 Rabbit mAb (A21149)

Western blot analysis of extracts of various cell lines, using CLIC4 antibody (A21149) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.