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PIK3C3/VPS34 Rabbit mAb (A12295)

Publications (2) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 Rabbit mAb (A12295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunofluorescence analysis of NIH-3T3 cells using PIK3C3/VPS34 Rabbit mAb (A12295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunoprecipitation analysis of 600 μg extracts of Rat brain cells using 3 μg PIK3C3/VPS34 antibody (A12295). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (A12295) at a dilution of 1:500.

Overview

Product namePIK3C3/VPS34 Rabbit mAb
Catalog No.A12295
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0286
Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 788-887 of human PIK3C3/VPS34 (Q8NEB9).
SequenceGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK
Gene ID
Swiss Prot
SynonymsVPS34; Vps34; hVps34
Calculated MW102kDa
Observed MW100kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • IP 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesRD, Mouse brain, Rat brain
Cellular locationCytoplasmic vesicle, Late endosome, Midbody, autophagosome
Customer validation

WB(Homo sapiens)

IHC(Rattus norvegicus)

Documents

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ABclonal:Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)}

Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 Rabbit mAb (A12295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)}

Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunofluorescence analysis of NIH-3T3 cells using PIK3C3/VPS34 Rabbit mAb (A12295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)}

Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunoprecipitation analysis of 600 μg extracts of Rat brain cells using 3 μg PIK3C3/VPS34 antibody (A12295). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (A12295) at a dilution of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

Related Targets

Based on existing publications, the following genes are closely related to research on PIK3C3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3C3. (Distance between topics and target gene indicate popularity.) PIK3C3

* Data provided by citexs.com, for reference only.

Publishing research using A12295? Please let us know so that we can cite the reference in this datasheet.