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PIK3C3/VPS34 Rabbit mAb (A12295)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 Rabbit mAb (A12295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunofluorescence analysis of NIH-3T3 cells using PIK3C3/VPS34 Rabbit mAb (A12295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug PIK3C3/VPS34 antibody (A12295). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (A12295) at a dilition of 1:500.

Overview

Product namePIK3C3/VPS34 Rabbit mAb
Catalog No.A12295
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 788-887 of human PIK3C3/VPS34 (Q8NEB9).
SequenceGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK
Gene ID
Swiss Prot
SynonymsVPS34; Vps34; hVps34
Calculated MW100kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesRD, Mouse brain, Rat brain
Cellular locationCytoplasmic vesicle, Late endosome, Midbody, autophagosome
ABclonal:Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)}

Western blot - PIK3C3/VPS34 Rabbit mAb (A12295)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 Rabbit mAb (A12295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)}

Immunofluorescence - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunofluorescence analysis of NIH-3T3 cells using PIK3C3/VPS34 Rabbit mAb (A12295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)}

Immunoprecipitation - PIK3C3/VPS34 Rabbit mAb (A12295)

Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug PIK3C3/VPS34 antibody (A12295). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (A12295) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.