Products > Antibodies > Monoclonal Antibodies

SOX4 Rabbit mAb (A21222)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOX4 Rabbit mAb (A21222)

Western blot analysis of extracts of various cell lines, using SOX4 antibody (A21222) at 1:22000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - SOX4 Rabbit mAb (A21222)

Immunofluorescence analysis of mouse brain using SOX4 Rabbit mAb (A21222) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameSOX4 Rabbit mAb
Catalog No.A21222
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.
ImmunogenRecombinant protein of human SOX4.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsSOX4; EVI16; SRY-box 4
Calculated MW47kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, SH-SY5Y, 293T, K-562, Mouse lung, Rat lung
Cellular locationNucleus
ABclonal:Western blot - SOX4 Rabbit mAb (A21222)}

Western blot - SOX4 Rabbit mAb (A21222)

Western blot analysis of extracts of various cell lines, using SOX4 antibody (A21222) at 1:22000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunofluorescence - SOX4 Rabbit mAb (A21222)}

Immunofluorescence - SOX4 Rabbit mAb (A21222)

Immunofluorescence analysis of mouse brain using SOX4 Rabbit mAb (A21222) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.