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ABScript II cDNA First-Strand Synthesis Kit (RK20400)

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Cat. No.SpecificationPrice
RK2040050 RXN$135.00
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RK20400100 RXN$255.00
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Description

ABScript II cDNA First Strand Synthesis Kit features two optimized mixes, ABScript II Enzyme Mix and ABScript II Reaction Mix. The enzyme mix combines ABScript II Reverse Transcriptase and RNase Inhibitor, and the reaction mix contains an optimized buffer. ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, which provides higher specificity and higher yield of cDNA.
The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.
Product Features

  • The low activity of RNase H is beneficial to the synthesis of long fragment cDNA, which can synthesize cDNA up to 10 KB.
  • The optimum temperature of the enzyme was 42⊥ and the enzyme still has high activity at 48⊥.
  • The specific cDNA yield was higher than that of ordinary M-MLV reverse transcriptase (37⊥).
  • Suitable for coronavirus detection;
  • With a variety of RT primers, both mRNA and ncRNA can be used as reverse transcription templates.
Applications

cDNA Synthesis, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis
Storage

-20°C

Product Information

Product Components

Components50 RXN100 RXN
ABScript II Enzyme Mix (10X)100 µL200 µL
ABScript II Reaction Mix (2X)600 µL1.25 mL
Oligo d(T)23VN (50 μM)100 µL200 µL
Random Primer Mix (60 μM)100 µL200 µL
dNTPs (10 mM each)50 µL100 µL
Nuclease-free H2O1.25 mL1.25 mL
ABScript II Enzyme Mix (10X)100 µL200 µL
Product Data

  • Using 1μg of HeLa cell total RNA as template, reverse transcription was performed with ABScript II cDNA First-Strand Synthesis Kit, and 1μL of cDNA was used as template to obtain clear and specific electrophoresis bands for genes of different lengths

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