Optional Antibody Services
ABclonal also offers a number of optional services to complement our antibody production services.
Antibody Expression
Expression Monoclonal Antibody Package Includes
- Gene synthesis – light chain and heavy chain
- Express and purify recombinant antibody
- Validate monoclonal antibody using ELISA
- Expand and purify antibody
Deliverables
- Antibodies purified from 1 L of culture fluid
- ELISA and other test reports
| Service |
Turnaround Time（weeks） |
Notes |
| Gene Synthesis |
Synthesis of light & heavy chain sequences |
1-2 |
- Antibody purity >90% SDS-PAGE is used to detect the purity of recombinant antibodies
- Antibody concentration: >0.5mg/mL A280 is used to detect the concentration of recombinant antibody
|
| Construction of antibody heavy and light chain sequences into recombinant antibody expression vectors |
1-2 |
| Sequence verification |
| Endotoxin-free plasmid purification |
| Vector Construction |
Transient transfection using HEK293/CHO cells |
2-3 |
| Recombinant antibody purification |
| Recombinant antibody quality control (SDS-PAGE and UV analysis) |
| Recombinant antibody validation(ELISA detection of immunogen) |
| Recombinant antibody large-scale expression |
Transient transfection using HEK293/CHO cells |
Further assessment is required |
| Screening of poly/monoclonal recombinant antibody expression stable transfected cell lines |
| Recombinant antibody large-scale production |
| Recombinant antibody purification |
| Recombinant antibody quality control (SDS-PAGE and UV analysis) |
| Recombinant antibody validation(ELISA detection of immunogen) |
Hybridoma Sequencing Service
- An antibody sequence is critical for monoclonal antibody engineering, antibody humanization, optimization of functionality, database cataloging, and antibody-related drug development. ABclonal provides customers with nothing but the highest-quality hybridoma sequencing services. Hybridoma sequencing mainly involves the sequencing of monoclonal antibodies’ variable regions.
Sequence Accuracy
Quality control
guaranteeing 100%
accuracy
Cost-Efficient
Competitive pricing
for excellent service
Fast Turnaround Time
On average,
15 business days
Broad Experience
Experienced in sequencing human, mouse, rat, and rabbit mAbs
- Starting from a hybridoma cell line, we offer the following services
Complete Package Details
Samples
- Freshly-harvested hybridoma cells
5 × 106 cells or more hybridoma cell lysate: entirely treated with TRIzol Reagent
Service Timeline
- Antibody isotyping identification
3-5 business days (alternatively, provided by customer)
Deliverables
- Detailed performance report
- Sequence data
- Alignments of heavy and light chains
- Plasmid containing V-region sequence
Note:
Please send us the samples as soon as possible using dry ice or liquid nitrogen. Delivery time will speed up significantly if customers provide the correct isotype and species information.
Other services
PeptidePeptide synthesis and conjugation / Peptide synthesis without conjugation / Peptide conjugation
Ascites Production ServiceFive mouse-scales of ascites production
LabelingBiotin, FITC, 5-FAM, rhodamine, HRP, etc.
